Interferon-gamma （IFN-γ） is a dimeric cytokine central to innate and adaptive immunity, primarily produced by activated T cells and natural killer （NK） cells. Structurally, IFN-γ exists as a homodimer, with each subunit comprising six α-helical domains stabilized by intra- and interchain disulfide bonds. It signals through a heterodimeric receptor complex composed of IFNGR1 and IFNGR2, initiating JAK-STAT pathway activation. Post-translational glycosylation at asparagine residues （e.g., Asn25 and Asn97 in humans） enhances its stability and modulates receptor interaction dynamics.
Immune Regulatory Roles and Therapeutic Cross-Talk of IFN-γ:
Interferon-gamma （IFN-γ） orchestrates multifaceted immune responses, exerting critical antiviral and antitumor defense by inducing cellular antiviral states and enhancing tumor immunosurveillance through upregulation of MHC class I/II expression. It activates macrophages to bolster phagocytosis, reactive oxygen species production, and pathogen clearance, while polarizing CD4+ T cells into Th1 subsets to amplify cell-mediated immunity. Despite its protective roles, IFN-γ exhibits a dual nature in inflammation, where excessive levels contribute to autoimmune disorders （e.g., rheumatoid arthritis, multiple sclerosis） and chronic inflammatory pathologies. Therapeutically, IFN-γ synergizes with proinflammatory cytokines like TNF-α and IL-12 to amplify immune activation, while counteracting the immunosuppressive effects of IL-4 and IL-10, highlighting its pivotal yet complex role in balancing immune defense and inflammatory regulation.
Advantages of this OneStep ELISA Kit Design:
Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time.
High Efficiency: Achieves results within 45 minutes, ideal for high-throughput studies or urgent diagnostics.
Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation.
Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design.
This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable IFN-γ quantification in time-sensitive or resource-limited settings.